Evaluation of Sorghum [Sorghumbicolor(L.)] Reference Genes in Various Tissues and under AbioticStress Conditions for Quantitative Real-Time PCRD at a Normalization

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cg.contactp.sudhakarreddy@cgiar.orgen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.crpCRP on Dryland Cereals - DCen_US
cg.contributor.funderNot Applicableen_US
cg.contributor.project-lead-instituteInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.coverage.countryINen_US
cg.coverage.regionSouthern Asiaen_US
cg.creator.idBhatnagar - Mathur, Pooja: 0000-0002-4150-8287en_US
cg.creator.idVadez, Vincent: 0000-0003-2014-0281en_US
cg.identifier.doihttps://dx.doi.org/10.3389/fpls.2016.00529en_US
cg.isijournalISI journalen_US
cg.issn1664-462Xen_US
cg.journalFrontiers in Plant Scienceen_US
cg.subject.agrovocagricultureen_US
cg.subject.agrovocsorghum bicoloren_US
cg.subject.agrovocqpcren_US
cg.subject.agrovocSorghumen_US
cg.volume07 (529en_US
dc.contributorReddy, Dumbala Srinivasen_US
dc.contributorKaliamoorthy, Sivasakthien_US
dc.contributorBhatnagar - Mathur, Poojaen_US
dc.contributorVadez, Vincenten_US
dc.contributorSharma, Kiranen_US
dc.creatorReddy, P. Sudhakaren_US
dc.date.accessioned2017-04-01T20:51:06Z
dc.date.available2017-04-01T20:51:06Z
dc.description.abstractAccurateandreliablegeneexpressiondatafromqPCRdependsonstablereferencegeneexpressionforpotentialgenefunctionalanalyses.Inthisstudy,15referencegeneswereselectedandanalyzedinvarioussamplesetsincludingabioticstresstreatments(salt,cold,waterstress,heat,andabscisicacid)andtissues(leaves,roots,seedlings,panicle,andmatureseeds).Statisticaltools,includinggeNorm,NormFinderandRefFinder,wereutilizedtoassessthesuitabilityofreferencegenesbasedontheirstabilityrankingsforvarioussamplegroups.Forabioticstress,PP2AandCYPwereidentifiedasthemoststablegenes.Incontrast,EIF4αwasthemoststableinthetissuesampleset,followedbyPP2A;PP2Awasthemoststableinallthesampleset,followedbyEIF4α.GAPDH,andUBC1weretheleaststablyexpressedinthetissueandallthesamplesets.Theseresultsalsoindicatedthattheuseoftwocandidatereferencegeneswouldbesufficientfortheoptimizationofnormalizationstudies.Tofurtherverifythesuitabilityofthesegenesforuseasreferencegenes,SbHSF5andSbHSF13geneexpressionlevelswerenormalizedusingthemostandleaststablesorghumreferencegenesinrootandwaterstressed-leaftissuesoffivesorghumvarieties.ThisisthefirstsystematicstudyoftheselectionofthemoststablereferencegenesforqPCR-relatedassaysinSorghumbicolorthatwillpotentiallybenefitfuturegeneexpressionstudiesinsorghumandothercloselyrelatedspecies.en_US
dc.formatPDFen_US
dc.identifierhttp://oar.icrisat.org/id/eprint/9449en_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/cl1jw8tq/v/e9f2c799fb1f79222c3b9679117532aeen_US
dc.identifier.citationP. Sudhakar Reddy, Dumbala Srinivas Reddy, Sivasakthi Kaliamoorthy, Pooja Bhatnagar - Mathur, Vincent Vadez, Kiran Sharma. (25/4/2016). Evaluation of Sorghum [Sorghumbicolor(L. )] Reference Genes in Various Tissues and under AbioticStress Conditions for Quantitative Real-Time PCRD at a Normalization. Frontiers in Plant Science, 07 (529, pp. 1-14.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6565
dc.languageenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceFrontiers in Plant Science;07 (529,(2016) Pagination 1-14en_US
dc.subjectreffinderen_US
dc.subjectgene expression stabilityen_US
dc.subjectreference geneen_US
dc.subjectnormalizationen_US
dc.titleEvaluation of Sorghum [Sorghumbicolor(L.)] Reference Genes in Various Tissues and under AbioticStress Conditions for Quantitative Real-Time PCRD at a Normalizationen_US
dc.typeJournal Articleen_US
dcterms.available2016-04-25en_US
dcterms.extent1-14en_US
mel.impact-factor4.495en_US

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