EST versus Genomic Derived Microsatellite Markers for Genotyping Wild and Cultivated Barley
| cg.contact | K.Chabane@cgiar.org | en_US |
| cg.contributor.center | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
| cg.contributor.center | Southern Cross University - SCU | en_US |
| cg.contributor.funder | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
| cg.contributor.project | Communication and Documentation Information Services (CODIS) | en_US |
| cg.contributor.project-lead-institute | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
| cg.date.embargo-end-date | Timeless | en_US |
| cg.identifier.doi | https://dx.doi.org/10.1007/s10722-003-6112-7 | en_US |
| cg.isijournal | ISI Journal | en_US |
| cg.issn | 0925-9864 | en_US |
| cg.issn | 1573-5109 | en_US |
| cg.issue | 7 | en_US |
| cg.journal | Genetic Resources and Crop Evolution | en_US |
| cg.subject.agrovoc | barley | en_US |
| cg.subject.agrovoc | Barley | en_US |
| cg.volume | 52 | en_US |
| dc.contributor | Ablett, G. A. | en_US |
| dc.contributor | Cordeiro, G. M. | en_US |
| dc.contributor | Valkoun, Jan | en_US |
| dc.contributor | Henry, R. J. | en_US |
| dc.creator | Chabane, K | en_US |
| dc.date.accessioned | 2021-03-24T23:16:19Z | |
| dc.date.available | 2021-03-24T23:16:19Z | |
| dc.description.abstract | Genetic variation present in wild and cultivated barley populations was investigated using two sources of microsatellite also known as simple sequence repeat (SSR) markers. EST-SSRs are derived from expressed sequences and genomic SSRs are isolated from genomic DNA. Genomic SSR markers detected a higher level of polymorphism than those derived from ESTs. Polymorphism information content was higher in genomic SSRs than EST-derived SSRs. This study showed that the EST-SSR markers developed in cultivated barley are polymorphic in wild and cultivated varieties and produced high quality markers. Ten of these functional markers were polymorphic across the accessions studied. EST markers indicated clearer separation between wild and cultivated barley than genomic SSRs. The EST-SSRs are a valuable source of new polymorphic markers and should be highly applicable to barley genetic resources, providing a direct estimate of functional biodiversity. | en_US |
| dc.identifier | https://mel.cgiar.org/dspace/limited | en_US |
| dc.identifier.citation | K Chabane, G. A. Ablett, G. M. Cordeiro, Jan Valkoun, R. J. Henry. (1/1/2005). EST versus Genomic Derived Microsatellite Markers for Genotyping Wild and Cultivated Barley. Genetic Resources and Crop Evolution, 52 (7), pp. 903-909. | en_US |
| dc.identifier.status | Timeless limited access | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.11766/12754 | |
| dc.language | en | en_US |
| dc.publisher | Springer (part of Springer Nature) | en_US |
| dc.source | Genetic Resources and Crop Evolution;52,(2005) Pagination 903-909 | en_US |
| dc.subject | simple sequence repeats (ssrs) | en_US |
| dc.subject | genetic diversity | en_US |
| dc.subject | expressed sequence tags derived microsatellites (est-ssrs) | en_US |
| dc.title | EST versus Genomic Derived Microsatellite Markers for Genotyping Wild and Cultivated Barley | en_US |
| dc.type | Journal Article | en_US |
| dcterms.available | 2005-01-01 | en_US |
| dcterms.extent | 903-909 | en_US |
| mel.impact-factor | 1.071 | en_US |