Transcriptome Analysis of a New Peanut Seed Coat Mutant for the Physiological Regulatory Mechanism Involved in Seed Coat Cracking and Pigmentation

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cg.contactlboshou@hotmail.comen_US
cg.contributor.centerChinese Academy of Agricultural Sciences, Oil Crops Research Institute - CAAS-OCRIen_US
cg.contributor.centerInternational Crops Research Institute for the Semi-Arid Tropics - ICRISATen_US
cg.contributor.centerChinese Academy of Agricultural Sciences, Institiute of Food Sciencea and Technology - CAAS-FOODCAASen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.countryCNen_US
cg.coverage.countryINen_US
cg.coverage.regionEastern Asiaen_US
cg.coverage.regionSouthern Asiaen_US
cg.creator.idPandey, Manish K: 0000-0002-4101-6530en_US
cg.identifier.doihttps://dx.doi.org/10.3389/fpls.2016.01491en_US
cg.isijournalISI journalen_US
cg.issnESSN: 1664-462Xen_US
cg.journalFrontiers in Plant Scienceen_US
cg.subject.agrovocagricultureen_US
cg.subject.agrovocpigmentationen_US
cg.volume7 (1491)en_US
dc.contributorLi, Beien_US
dc.contributorPandey, Manish Ken_US
dc.contributorWu, Yanshanen_US
dc.contributorLei, Yongen_US
dc.contributorYan, Liyingen_US
dc.contributorDai, Xiaofengen_US
dc.contributorJiang, Huifangen_US
dc.contributorZhang, Junchengen_US
dc.contributorWei, Guoen_US
dc.contributorVarshney, Rajeeven_US
dc.contributorLiao, Boshouen_US
dc.creatorwan, liyunen_US
dc.date.accessioned2017-04-26T21:15:38Z
dc.date.available2017-04-26T21:15:38Z
dc.description.abstractSeed-coatcrackingandundesirablecolorofseedcoathighlyaffectsexternalappearanceandcommercialvalueofpeanuts(ArachishypogaeaL.).Withanobjectivetofindgeneticsolutiontotheaboveproblems,apeanutmutantwithcrackingandbrowncoloredseedcoat(testa)wasidentifiedfromanEMStreatedmutantpopulationanddesignatedas“peanutseedcoatcrackandbrowncolormutantline(pscb).”Theseedcoatweightofthemutantwasalmosttwiceofthewildtype,andthegerminationtimewassignificantlyshorterthanwildtype.Further,themutanthadlowerleveloflignin,anthocyanin,proanthocyanidincontent,andhighlyincreasedlevelofmelanincontentascomparedtowildtype.UsingRNA-Seq,weexaminedtheseedcoattranscriptomeinthreestagesofseeddevelopmentinthewildtypeandthepscbmutant.TheRNA-Seqanalysisrevealedpresenceofhighlydifferentiallyexpressedphenylpropanoidandflavonoidpathwaygenesinallthethreeseeddevelopmentstges,especiallyat40daysafterflowering(DAF40).Also,theexpressionofpolyphenoloxidasesandperoxidasewerefoundtobeactivatedsignificantlyespeciallyinthelateseeddevelopmentalstage.Thegenome-widecomparativestudyoftheexpressionprofilesrevealed62differentiallyexpressedgenescommonacrossallthethreestages.Byanalyzingtheexpressionpatternsandthesequencesofthecommondifferentiallyexpressedgenesofthethreestages,threecandidategenesnamelyc36498_g1(CCoAOMT1),c40902_g2(kinesin),andc33560_g1(MYB3)wereidentifiedresponsibleforseed-coatcrackingandbrowncolorphenotype.Therefore,thisstudynotonlyprovidedcandidategenesbutalsoprovidedgreaterinsightsandmoleculargeneticcontrolofpeanutseed-coatcrackingandcolorvariation.Theinformationgeneratedinthisstudywillfacilitatefurtheridentificationofcausalgeneanddiagnosticmarkersforbreedingimprovedpeanutvrietieswithsmoothanddesirableseedcoatcolor.en_US
dc.formatPDFen_US
dc.identifierhttp://oar.icrisat.org/id/eprint/9838en_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/w2dtVnfX/v/c40311aa3e72352b5c7ea788b22585a7en_US
dc.identifier.citationLiyun wan, Bei Li, Manish K Pandey, Yanshan Wu, Yong Lei, Liying Yan, Xiaofeng Dai, Huifang Jiang, Juncheng Zhang, Guo Wei, Rajeev Varshney, Boshou Liao. (31/10/2016). Transcriptome Analysis of a New Peanut Seed Coat Mutant for the Physiological Regulatory Mechanism Involved in Seed Coat Cracking and Pigmentation. Frontiers in Plant Science, 7 (1491), pp. 1-15.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/6892
dc.languageenen_US
dc.publisherFrontiers Mediaen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceFrontiers in Plant Science;7 (1491),(2016) Pagination 1,15en_US
dc.subjectrna-seqen_US
dc.subjectpeanut (arachishypogaea)en_US
dc.subjectseed-coatcrackingen_US
dc.subjectflavonoidpathwayen_US
dc.subjectPeanuten_US
dc.titleTranscriptome Analysis of a New Peanut Seed Coat Mutant for the Physiological Regulatory Mechanism Involved in Seed Coat Cracking and Pigmentationen_US
dc.typeJournal Articleen_US
dcterms.available2016-10-31en_US
dcterms.extent1-15en_US
mel.impact-factor4.495en_US

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