Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)

cg.contactsisobe@kazusa.or.jpen_US
cg.contributor.centerKazusa DNA Research Instituteen_US
cg.contributor.centerAgricultural Research Center, Field Crops Research Institute - ARC - FCRIen_US
cg.contributor.crpCGIAR Research Program on Dryland Systems - DSen_US
cg.contributor.crpCGIAR Research Program on Grain Legumes - GLen_US
cg.contributor.crpCGIAR Research Program on Wheat - WHEATen_US
cg.contributor.funderInternational Fund for Agricultural Development - IFADen_US
cg.contributor.projectEnhanced small-holder wheat-legume cropping systems to improve food security under changing climate in the drylands of West Asia and North Africaen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.identifier.doihttps://dx.doi.org/10.1270/jsbbs.64.252en_US
cg.isijournalISI Journalen_US
cg.issn1344-7610en_US
cg.journalBreeding Scienceen_US
cg.subject.agrovocfaba beansen_US
cg.volume64en_US
dc.contributorKimura, Mitsuhiroen_US
dc.contributorHirakawa, Hidekien_US
dc.contributorSabah, Attiaen_US
dc.contributorShirasawa, Kentaen_US
dc.contributorSato, Shuseien_US
dc.contributorTabata, Satoshien_US
dc.contributorSasamoto, Shigemien_US
dc.contributorWatanabe, Akikoen_US
dc.contributorKawashima, Kumikoen_US
dc.contributorKato, Midorien_US
dc.contributorWada, Tsuyukoen_US
dc.contributorTsuruoka, Hisanoen_US
dc.contributorTakahashi, Chikaen_US
dc.contributorMinami, Chiharuen_US
dc.contributorNanri, Keikoen_US
dc.contributorNakayama, Shinobuen_US
dc.contributorKohara, Mitsuyoen_US
dc.contributorYamada, Manabuen_US
dc.contributorKishida, Yoshieen_US
dc.contributorFujishiro, Tsunakazuen_US
dc.contributorIsobe, Sachikoen_US
dc.creatorEl-Rodeny, Waliden_US
dc.date.accessioned2017-11-29T13:00:31Z
dc.date.available2017-11-29T13:00:31Z
dc.description.abstractTo develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]­derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a ‘Nubaria 2’ × ‘Misr 3’ F2 mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 F2 plants were divided into three subpopulations according to the original F1 plants. Linkage groups (LGs) generated in each subpopulation were integrated by commonly mapped markers. The integrated ‘Nubaria 2’ × ‘Misr 3’ map consisted of six LGs, representing a total length of 684.7 cM, with 552 loci. Of the mapped loci, 47% were generated from multi-loci diagnostic (MLD) markers. Alignment of homologous sequence pairs along each linkage group revealed obvious syntenic relationships between LGs in faba bean and the genomes of two model legumes, Lotus japonicus and Medicago truncatula. In a polymorphic analysis with ten Egyptian faba bean varieties, 78.9% (384/487) of the FBES markers showed polymorphisms. Along with the EST-SSR markers, the dense map developed in this study is expected to accelerate marker assisted breeding in faba bean.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/N8cmWRXs/v/0850c14741b9059b8f86e515658de63aen_US
dc.identifier.citationWalid El-Rodeny, Mitsuhiro Kimura, Hideki Hirakawa, Attia Sabah, Kenta Shirasawa, Shusei Sato, Satoshi Tabata, Shigemi Sasamoto, Akiko Watanabe, Kumiko Kawashima, Midori Kato, Tsuyuko Wada, Hisano Tsuruoka, Chika Takahashi, Chiharu Minami, Keiko Nanri, Shinobu Nakayama, Mitsuyo Kohara, Manabu Yamada, Yoshie Kishida, Tsunakazu Fujishiro, Sachiko Isobe. (24/6/2014). Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba). Breeding Science, 64, pp. 252-263.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/7548
dc.languageenen_US
dc.publisherJapanese Society of Breedingen_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceBreeding Science;64,(2014) Pagination 252-263en_US
dc.subjectest-ssren_US
dc.subjectlinkage mapen_US
dc.subjectcomparative mappingen_US
dc.subjectgenetic diversityen_US
dc.titleDevelopment of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)en_US
dc.typeJournal Articleen_US
dcterms.available2014-06-24en_US
dcterms.extent252-263en_US
mel.impact-factor1.559en_US
mel.project.openhttps://mel.cgiar.org/projects/46en_US

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