Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU

cg.contactS.KUMARI@CGIAR.ORGen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.crpResilient Agrifood Systems - RAFSen_US
cg.contributor.crpGenetic Innovation - GIen_US
cg.contributor.funderCGIAR Trust Funden_US
cg.contributor.initiativeGenebanksen_US
cg.contributor.initiativePlant Healthen_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryLBen_US
cg.coverage.end-date2022-12-01en_US
cg.coverage.regionWestern Asiaen_US
cg.creator.idKumari, Safaa: 0000-0002-4492-6257en_US
cg.creator.idMoukahel, Abdulrahman: 0000-0003-4297-771Xen_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.agrovocfood securityen_US
cg.subject.agrovocmolecular characterizationen_US
cg.subject.agrovocvirus diseasesen_US
cg.subject.agrovocplant healthen_US
cg.subject.agrovocFaba beanen_US
cg.subject.agrovocLentilen_US
cg.subject.agrovocChickpeaen_US
cg.subject.agrovocGrass pea (Lathyrus sativus)en_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 1 - No povertyen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
dc.contributorMoukahel, Abdulrahmanen_US
dc.creatorKumari, Safaaen_US
dc.date.accessioned2023-01-24T03:03:42Z
dc.date.available2023-01-24T03:03:42Z
dc.description.abstractPea seed-borne mosaic virus (PSbMV, genus Potyvirus, family Potyviridae) is the most important virus infecting lentil both in terms of economic importance and geographical distribution. It was reported that PSbMV can even lead up to 61% yield loss. Therefore, developing and implementing effective management strategies for PSbMV is very important to reduce yield and quality loss. However, accurate detection of virus disease is the first essential step for effective management strategies for control of this disease. Molecular is efficient tool to accurately detect and monitor the occurrence and development of crop diseases. This report describes a validated reverse transcription-polymerase chain reaction (RT-PCR) assay for PSbMV detection and characterization applied by ICARDA’s GHU.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/981a150c003fe1c0bac07f5fd7a6cd47/v/7b607c443270da43d15b6170aa487e39en_US
dc.identifier.citationSafaa G. Kumari and Abdelrahman Moukahel. 2022. Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHU. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/68025
dc.languageenen_US
dc.publisherInternational Center for Agricultural Research in the Dry Areas (ICARDA)en_US
dc.rightsCC-BY-SA-4.0en_US
dc.subjectseed health testingen_US
dc.subjectseed-borne virusen_US
dc.subjectField pea (Pisum sativum)en_US
dc.titleReverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Pea seed-borne mosaic virus in legumes applied by ICARDA’s GHUen_US
dc.typeToolen_US
dcterms.available2022-12-01en_US
dcterms.issued2022-12-01en_US

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