Molecular identification of chlamydial cause of abortion in small ruminants in Jordan

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cg.contacthothaifaababneh@yahoo.comen_US
cg.contributor.centerNational Agricultural Research Center (National Center for Agricultural Research and Extension) - NARC (NCARE)en_US
cg.contributor.centerJordan University of Science and Technology - JUSTen_US
cg.contributor.crpCRP on Dryland Systems - DSen_US
cg.contributor.funderNot Applicableen_US
cg.coverage.countryJOen_US
cg.coverage.regionWestern Asiaen_US
cg.date.embargo-end-date2017-01-31en_US
cg.identifier.doihttps://dx.doi.org/10.1007/s11250-014-0654-xen_US
cg.isijournalISI journalen_US
cg.issue8en_US
cg.journalTropical animal health and productionen_US
cg.subject.agrovocsmall ruminantsen_US
cg.subject.agrovocchlamydophila abortusen_US
cg.subject.agrovocjordanen_US
cg.volume46en_US
dc.contributorAbabneh, Mustafaen_US
dc.contributorHananeh, Wael M.en_US
dc.contributorAl-Sheyab, Fawzien_US
dc.contributorJawasreh, Khaleel I.en_US
dc.contributorAl-Gharaibeh, Moathen_US
dc.contributorAbabneh, Mohammeden_US
dc.creatorAbabneh, Hutheifaen_US
dc.date.accessioned2017-01-10T00:28:35Z
dc.date.available2017-01-10T00:28:35Z
dc.description.abstractChlamydophila abortus (Ch. abortus) is the etiological agent of ovine enzootic abortion (OEA) and one of the most common infectious agents of abortion in small ruminants worldwide. RFLP-PCR analysis of the outer membrane protein gene (OMP2 gene) was used for diagnosis and characterization of chlamydial causes of abortion in small ruminants in Jordan. Sixty-six placental tissues and 15 vaginal swabs were collected from aborted ewes and does to identify cause of abortion in Jordan. Thirty-eight placental samples (58 %) and 13 vaginal swabs (87 %) were positive for chlamydial DNA. Shedding of bacteria in vaginal swabs was detected within 7 days after abortion. The results of this study showed that chlamydiosis is one of the important causes of abortion in small ruminants in Jordan. In addition, vaginal swab is an excellent sample for molecular diagnosis of chlamydiosis. DNA sequencing and RFLP analysis of the OMP2 reveal that all chlamydial cause of abortion in small ruminants in Jordan are due to Ch. abortus. While, Ch. pecorum was not detected in any sample. OMP2 gene of the isolated Jordanian strain was identical (100 %) to Ch. abortus FAS strain. In conclusion, Ch. abortus is an important cause of abortion in Jordan; vaginal swab within 7 days of abortion can be used for molecular diagnosis of chlamydiosis in small ruminants.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/Sjkoa0qP/v/4f39782335ff5ebbf96fcfde3d22c9b6en_US
dc.identifier.citationHutheifa Ababneh, Mustafa Ababneh, Wael M. Hananeh, Fawzi Al-Sheyab, Khaleel I. Jawasreh, Moath Al-Gharaibeh, Mohammed Ababneh. (12/8/2014). Molecular identification of chlamydial cause of abortion in small ruminants in Jordan. Tropical animal health and production, 46(8), pp. 1407-1412.en_US
dc.identifier.statusLimited accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/5450
dc.languageenen_US
dc.publisherSpringer Verlag (Germany)en_US
dc.rightsCC-BY-NC-4.0en_US
dc.sourceTropical animal health and production;46,(2014) Pagination 1407,1412en_US
dc.subjectovine enzootic abortionen_US
dc.subjectouter membrane protein geneen_US
dc.subjectpcr-rflpen_US
dc.subjectdna sequencingen_US
dc.titleMolecular identification of chlamydial cause of abortion in small ruminants in Jordanen_US
dc.typeJournal Articleen_US
dcterms.available2014-08-12en_US
dcterms.extent1407-1412en_US

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