Identification of barley mutants in the cultivar 'Lux' at the Dhn loci through TILLING

cg.contactm.baum@cgiar.orgen_US
cg.contributor.centerInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.centerUniversity of Aleppoen_US
cg.contributor.centerUniversity of Copenhagen, Faculty of Science - KU - Scienceen_US
cg.contributor.funderInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.contributor.projectCommunication and Documentation Information Services (CODIS)en_US
cg.contributor.project-lead-instituteInternational Center for Agricultural Research in the Dry Areas - ICARDAen_US
cg.coverage.countryDKen_US
cg.coverage.regionNorthern Europeen_US
cg.creator.idBaum, Michael: 0000-0002-8248-6088en_US
cg.identifier.doihttps://dx.doi.org/10.1111/j.1439-0523.2009.01640.xen_US
cg.isijournalISI Journalen_US
cg.issn0179-9541en_US
cg.issn1439-0523en_US
cg.issue4en_US
cg.journalPlant Breedingen_US
cg.subject.agrovocbarleyen_US
cg.subject.agrovochordeum vulgareen_US
cg.subject.agrovoctillingen_US
cg.volume128en_US
dc.contributorMejlhede, N.en_US
dc.contributorRasmussen, S.en_US
dc.contributorBackes, Gunteren_US
dc.contributorAl-Said, W.en_US
dc.contributorBaum, Michaelen_US
dc.contributorJahoor, Ahmeden_US
dc.creatorLababidi, Sameren_US
dc.date.accessioned2021-01-14T21:03:25Z
dc.date.available2021-01-14T21:03:25Z
dc.description.abstractTILLING is a reverse genetic strategy that allows screening for mutations in genes with known sequences in a plant mutant population. A TILLING population has been developed for the Danish barley variety 'Lux' (Hordeum vulgare L.), by using sodium azide to induce mutations. Scoring of four visible phenotypic characters of barley seedling in reference to the parental cultivar 'Lux' in the M-3 plants showed over 3.5% lethality. A series of pool ratios of mixed DNA from mutant lines were tested and 10-fold pools appeared to be the practical mixing ratio for the detection of fragments in the 500-700 bp range. Two of the 13 known dehydrin genes, Dhn12 and Dhn13, respectively, were examined and five independent missense mutations were obtained from a population of 9575 barley mutant plants. This corresponds to a mutation density of approximately one mutation every two and half million base pairs for these two genes. The mutant population of approximately 10 000 lines was screened for mutations in two genes in a short time due to high pooling ratio.en_US
dc.formatPDFen_US
dc.identifierhttps://mel.cgiar.org/reporting/downloadmelspace/hash/9944477847b43b17f3e8d72f8252410a/v/a0476a7fbfc2d9861a1a7b41324fc4d8en_US
dc.identifier.citationSamer Lababidi, N. Mejlhede, S. Rasmussen, Gunter Backes, W. Al-Said, Michael Baum, Ahmed Jahoor. (3/8/2009). Identification of barley mutants in the cultivar 'Lux' at the Dhn loci through TILLING. Plant Breeding, 128 (4), pp. 332-332.en_US
dc.identifier.statusOpen accessen_US
dc.identifier.urihttps://hdl.handle.net/20.500.11766/12357
dc.languageenen_US
dc.publisherWiley (12 months)en_US
dc.rightsCopyrighted; Non-commercial educational use onlyen_US
dc.sourcePlant Breeding;128,(2009) Pagination 332-332en_US
dc.subjectdehydrinen_US
dc.subjectdhn12en_US
dc.subjectdhn13en_US
dc.titleIdentification of barley mutants in the cultivar 'Lux' at the Dhn loci through TILLINGen_US
dc.typeJournal Articleen_US
dcterms.available2009-08-03en_US
dcterms.extent332-332en_US
dcterms.issued2009-08-01en_US
mel.impact-factor1.662en_US

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