Mapping QTL Associated with Traits Affecting Grain Yield in Chickpea (Cicer arietinum L.) under Terminal Drought Stress

Abstract

The aim of this study was to evaluate a set of recombinant inbred lines (RILs) for agronomic and physiological traits under drought conditions and to locate quantitative trait loci (QTL) associated with them. This study used a RIL population derived from a cross between drought tolerant (ILC 588) and susceptible (ILC 3279) genotypes. The population consisting of 155 RILs was grown under drought conditions in the field at Tel Hadya, Syria, in 2006 and 2007 and at Breda, Syria, in 2007. A genetic map consisting of eight linkage groups was developed using 97 simple sequence repeat (SSR) markers. The results revealed that high harvest index (HI), early flowering, and early maturity were the important attributes contributing to higher grain yield under drought. Higher stomatal conductance (gs) and cooler canopies (canopy temperature minus air temperature [Tc–Ta]) can also lead to better performance under drought conditions. Quantitative trait locus analysis identified 15 genomic regions significantly associated with various traits affecting drought tolerance in chickpea. Important QTL detected in this study included two QTL for HI explaining 38% of the total phenotypic variability of the trait, four QTL for flowering explaining 45%, and three QTL for maturity explaining 52% on a cumulative basis. Three QTL for gs and six QTL for Tc–Ta also detected explained 7 to 15% phenotypic variability individually. Two QTL (Q3‐1 and Q1‐1) on linkage group 3 (LG3) and LG1 showed effects on many traits related to drought. Hence, these regions can be further explored in future drought studies.