Development of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis under Excess Iron Stress
cg.contact | kevin.mcphee@ndsu.edu | en_US |
cg.contributor.center | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.contributor.center | Indian Council of Agricultural Research, Indian Institute of Pulses Research - ICAR-IIPR | en_US |
cg.contributor.center | North Dakota State University - NDSU | en_US |
cg.contributor.crp | CGIAR Research Program on Grain Legumes - GL | en_US |
cg.contributor.funder | Arab Fund for Economic and Social Development - AFESD | en_US |
cg.contributor.project | Sustainability and Operation of the Regional Research Centers in a Number of Arab Countries (Phase II) | en_US |
cg.contributor.project-lead-institute | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.creator.id | Agrawal, Shiv Kumar: 0000-0001-8407-3562 | en_US |
cg.identifier.doi | https://dx.doi.org/10.3389/fpls.2017.00579 | en_US |
cg.isijournal | ISI Journal | en_US |
cg.issn | 1664-462X | en_US |
cg.journal | Frontiers in Plant Science | en_US |
cg.subject.agrovoc | gene expression | en_US |
cg.subject.agrovoc | lentils | en_US |
cg.subject.agrovoc | ferritin | en_US |
cg.subject.agrovoc | lentil | en_US |
cg.volume | 8:579 | en_US |
dc.contributor | McPhee, Kevin | en_US |
dc.contributor | Agrawal, Shiv Kumar | en_US |
dc.creator | Sen Gupta, Debjyoti | en_US |
dc.date.accessioned | 2018-03-07T00:59:45Z | |
dc.date.available | 2018-03-07T00:59:45Z | |
dc.description.abstract | Multiple genes and transcription factors are involved in the uptake and translocation of iron in plants from soil. The sequence information about iron uptake and translocation related genes is largely unknown in lentil (Lens culinaris Medik.). This study was designed to develop iron metabolism related molecular markers for Ferritin-1, BHLH-1 (Basic helix loop helix), or FER-like transcription factor protein and IRT-1 (Iron related transporter) genes using genome synteny with barrel medic (Medicago truncatula). The second objective of this study was to analyze differential gene expression under excess iron over time (2 h, 8 h, 24 h). Specific molecular markers were developed for iron metabolism related genes (Ferritin-1, BHLH-1, IRT-1) and validated in lentil. Gene specific markers for Ferritin-1 and IRT-1 were used for quantitative PCR (qPCR) studies based on their amplification efficiency. Significant differential expression of Ferritin-1 and IRT-1 was observed under excess iron conditions through qPCR based gene expression analysis. Regulation of iron uptake and translocation in lentil needs further characterization. Greater emphasis should be given to development of conditions simulating field conditions under external iron supply and considering adult plant physiology. | en_US |
dc.format | en_US | |
dc.identifier | https://www.frontiersin.org/articles/10.3389/fpls.2017.00579/full | en_US |
dc.identifier | https://www.researchgate.net/publication/315705264_Development_of_Molecular_Markers_for_Iron_Metabolism_Related_Genes_in_Lentil_and_Their_Expression_Analysis_under_Excess_Iron_Stress | en_US |
dc.identifier | https://mel.cgiar.org/reporting/downloadmelspace/hash/MoFWuv50/v/3082596d4959e2a544ca8c6bd24b4850 | en_US |
dc.identifier.citation | Debjyoti Sen Gupta, Kevin McPhee, Shiv Kumar Agrawal. (13/4/2017). Development of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis under Excess Iron Stress. Frontiers in Plant Science, 8: 579. | en_US |
dc.identifier.status | Open access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/8011 | |
dc.language | en | en_US |
dc.publisher | Frontiers Media | en_US |
dc.rights | CC-BY-4.0 | en_US |
dc.source | Frontiers in Plant Science;8:579,(2017) | en_US |
dc.subject | molecular marker | en_US |
dc.subject | iron metabolism | en_US |
dc.subject | qpcr expression analysis | en_US |
dc.title | Development of Molecular Markers for Iron Metabolism Related Genes in Lentil and Their Expression Analysis under Excess Iron Stress | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2017-04-13 | en_US |
mel.impact-factor | 3.678 | en_US |
mel.project.open | https://mel.cgiar.org/projects/216 | en_US |