FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep
cg.contact | bedhiaf.sonia@gmail.com | en_US |
cg.contributor.center | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.contributor.center | National Agricultural Research Institute of Tunisia - INRAT | en_US |
cg.contributor.center | Manouba University, National School of Veterinary Medicine of Sidi Thabet - ENMV | en_US |
cg.contributor.center | University of Carthage - UCAR Tunis | en_US |
cg.contributor.center | University of Toulouse - Univ Toulouse | en_US |
cg.contributor.center | Manouba University - UMA | en_US |
cg.contributor.crp | CGIAR Research Program on Livestock and Fish - L&F | en_US |
cg.contributor.funder | International Livestock Research Institute - ILRI | en_US |
cg.contributor.project | CGIAR Research Program on Livestock Agri-Food Systems | en_US |
cg.contributor.project-lead-institute | International Center for Agricultural Research in the Dry Areas - ICARDA | en_US |
cg.coverage.country | TN | en_US |
cg.coverage.region | Northern Africa | en_US |
cg.creator.id | Rekik, Mourad: 0000-0001-7455-2017 | en_US |
cg.identifier.doi | https://dx.doi.org/10.1186/s12863-017-0510-x | en_US |
cg.isijournal | ISI Journal | en_US |
cg.issn | 1471-2156 | en_US |
cg.journal | BMC Genetics | en_US |
cg.subject.agrovoc | sheep | en_US |
cg.subject.agrovoc | barbarine | en_US |
cg.subject.agrovoc | ovulation | en_US |
cg.volume | 18: 43 | en_US |
dc.contributor | Benkhlil, Zohra | en_US |
dc.contributor | Woloszyn, Florent | en_US |
dc.contributor | Rejeb, Ahmed | en_US |
dc.contributor | Aouina, Mohamed | en_US |
dc.contributor | Rekik, Mourad | en_US |
dc.contributor | Fabre, Stephane | en_US |
dc.contributor | Bedhiaf, Sonia | en_US |
dc.creator | Lassoued, Narjess | en_US |
dc.date.accessioned | 2018-02-07T09:10:57Z | |
dc.date.available | 2018-02-07T09:10:57Z | |
dc.description.abstract | Background: Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the “W” flock selected for improved prolificacy, we found prolific and infertile ewes with streaky ovaries. Blood genomic DNA was extracted from a subset of low-ovulating, prolific and infertile ewes of the “W” flock, and the entire coding sequences of GDF9 and BMP15 were sequenced. Results: We evidenced a novel polymorphism in the exon 1 of the BMP15 gene associated with increased prolificacy and sterility. This novel mutation called FecXBar is a composite polymorphism associating a single nucleotide substitution (c.301G > T), a 3 bp deletion (c.302_304delCTA) and a C insertion (c.310insC) in the ovine BMP15 cDNA leading to a frame shift at protein position 101. Calculated in the “W” flock, the FecXBar allele increased OR by 0.7 ova and LS by 0.3 lambs (p = 0.08). As for already identified mutations, homozygous females carrying FecXBar exhibited streaky ovaries with a blockade at the primary stage of folliculogenesis as shown by histochemistry. Conclusions: Our investigation demonstrates a new mutation in the BMP15 gene providing a valuable genetic tool to control fecundity in Tunisian Barbarine, usable for diffusion program into conventional flocks looking for prolificacy improvement. | en_US |
dc.format | en_US | |
dc.identifier | https://bmcgenet.biomedcentral.com/articles/10.1186/s12863-017-0510-x | en_US |
dc.identifier | https://www.researchgate.net/publication/317009926_FecX_a_Novel_BMP15_mutation_responsible_for_prolificacy_and_female_sterility_in_Tunisian_Barbarine_Sheep | en_US |
dc.identifier | https://mel.cgiar.org/reporting/downloadmelspace/hash/4N1vEtux/v/5dbf5e5e55c10cf5adee7eeb748c53f3 | en_US |
dc.identifier.citation | Narjess Lassoued, Zohra Benkhlil, Florent Woloszyn, Ahmed Rejeb, Mohamed Aouina, Mourad Rekik, Stephane Fabre, Sonia Bedhiaf. (15/5/2017). FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep. BMC Genetics, 18: 43. | en_US |
dc.identifier.status | Open access | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.11766/7792 | |
dc.language | en | en_US |
dc.publisher | BioMed Central | en_US |
dc.rights | CC-BY-4.0 | en_US |
dc.source | BMC Genetics;18: 43,(2017) | en_US |
dc.subject | bmp15 | en_US |
dc.subject | major gene | en_US |
dc.subject | prolificacy | en_US |
dc.title | FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep | en_US |
dc.type | Journal Article | en_US |
dcterms.available | 2017-05-15 | en_US |
mel.impact-factor | 2.266 | en_US |
mel.project.open | https://mel.cgiar.org/projects/237 | en_US |