Njaci, IsaacSarkar, AbhimanyuJiang, ZhouqianKaithakottil, GemyMoore, ChristopherCheema, JitenderStevenson, Clare E. M.Rejzek, MartinNovak, PetrVigouroux, MarielleVickers, MartinWouters, Roland H. MPaajanen, PiritaSteuernagel, BurkhardMoore, Jonathan DHiggins, JanetSwarbreck, DavidMartens, StefanKim, Colin YWeng, Jing-KeMundree, SagadevanKilian, BenjaminAgrawal, Shiv KumarLoose, MattYant, LeviMacas, JiříWang, Trevor L.Martin, CathieEmmrich, Peter M. F.2023-10-042023-10-04Anne Edwards, Isaac Njaci, Abhimanyu Sarkar, Zhouqian Jiang, Gemy Kaithakottil, Christopher Moore, Jitender Cheema, Clare E. M. Stevenson, Martin Rejzek, Petr Novak, Marielle Vigouroux, Martin Vickers, Roland H. M Wouters, Pirita Paajanen, Burkhard Steuernagel, Jonathan D Moore, Janet Higgins, David Swarbreck, Stefan Martens, Colin Y Kim, Jing-Ke Weng, Sagadevan Mundree, Benjamin Kilian, Shiv Kumar Agrawal, Matt Loose, Levi Yant, Jiří Macas, Trevor L. Wang, Cathie Martin, Peter M. F. Emmrich. (16/2/2023). Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus. Nature Communications, 14.https://hdl.handle.net/20.500.11766/68722Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin.PDFCC-BY-4.0biochemical analysesβ-l-odap biosynthesisJournal ArticleOpen access