Chen, HuataoHien, Vu Thi ThuHamwieh, AladdinYamada, TetsuyaSato, TadashiYan, YongliangCong, HuaShono, MarikoSuenaga, KazuhiroXu, D. H.2022-02-152022-02-15Do Duc Tuyen, Huatao Chen, Vu Thi Thu Hien, Aladdin Hamwieh, Tetsuya Yamada, Tadashi Sato, Yongliang Yan, Hua Cong, Mariko Shono, Kazuhiro Suenaga, D. H. Xu. (8/1/2016). Ncl Synchronously Regulates Na+, K+, and Cl− in Soybean and Greatly Increases the Grain Yield in Saline Field Conditions. Scientific Reports, 6.https://hdl.handle.net/20.500.11766/67058Salt stress inhibits soybean growth and reduces gain yield. Genetic improvement of salt tolerance is essential for sustainable soybean production in saline areas. In this study, we isolated a gene (Ncl) that could synchronously regulate the transport and accumulation of Na+, K+, and Cl-from a Brazilian soybean cultivar FT-Abyara using map-based cloning strategy. Higher expression of the salt tolerance gene Ncl in the root resulted in lower accumulations of Na+, K+, and Cl-in the shoot under salt stress. Transfer of Ncl with the Agrobacterium-mediated transformation method into a soybean cultivar Kariyutaka significantly enhanced its salt tolerance. Introgression of the tolerance allele into soybean cultivar Jackson, using DNA marker-assisted selection (MAS), produced an improved salt tolerance line. Ncl could increase soybean grain yield by 3.6-5.5 times in saline field conditions. Using Ncl in soybean breeding through gene transfer or MAS would contribute to sustainable soybean production in salineprone areas.PDFCC-BY-4.0saltfield conditionsJournal ArticleOpen access